Ukubeka iliso ekuvuthweni kunye nolawulo lwenkqubo kuthetha ukudala indawo egqibeleleyo yokukhula kunye nokuvutshelwa kweentsholongwane, Emva koko kugcina ukusebenza kakuhle okuzinzileyo, isivuno kunye nomgangatho wemveliso ohambelanayo. Iiparameter eziphambili ezifana ne-pH, ioksijini enyibilikisiweyo, ubushushu, uxinano lwe-substrate, kunye ne-viscosity zonke zezi zinto ezimisela umgangatho kunye nokusebenza kakuhle kwiifektri zamayeza, apho iimfuno ezingqongqo zibekwa phambili kumgca wenkqubo.
Iimfuneko zokuBilisa iBroth Viscosity kunye nokuJonga ukuQonda
Zombini uxinaniso kunye noxinaniso zisebenza njengophawu olutshintshayo lokukhula kweentsholongwane, uxinano lweeseli, kunye nokwakheka komhluzi, zichaphazela kakhulu izinto ezibalulekileyo ezifana nokudluliselwa kobunzima, ukufumaneka kweoksijini, kunye nokusasazwa kwezondlo. Ngaphandle kokujonga ngokucokisekileyo ukubila kunye nokulawula inkqubo, ukuguquguquka koxinaniso kunye noxinaniso kunokuphazamisa ulungelelwaniso olubuthathaka lwendawo yokubila, okukhokelela ekunciphiseni isivuno, umgangatho wemveliso ongaguqukiyo, kunye nokungasebenzi kakuhle kwexabiso.
Ukubeka esweni rhoqo inkqubo yokubilisa kwi-intanethi kubonelela ngedatha yexesha langempela, nto leyo enika amandla abaqhubi ukuba benze uhlengahlengiso olukhawulezileyo noluchanekileyo kwiiparameter ezifana namazinga okutya okunesondlo kunye namanqanaba omoya. Kususwe iziphumo ezimbi ezibangelwa kukuthatha isampuli ngesandla okulibazisekayo nokuthatha ixesha.
Isishwankathelo seNkqubo yokuBilisa iPenicillium
Ukubiliswa kwamayeza yiprojekthi enobuchule ye-microbiology kunye nobunjineli, apho iintsholongwane ezikhuliswe ngononophelo, ezifana neebhaktheriya okanye iifungi, zivelisa iimveliso ezibalulekileyo zebhayoloji ezifana nee-antibiotics, ii-vaccines, kunye nee-enzymes.
Inkqubo yokubilisa iPenicillium iqala ngokulungiswa kwe-inoculum kwiitanki zembewu. Emva koko itshintshele kwi-feed-batch fermentation kwi-media etyebiswe ngezondlo ngaphakathi kwe-fermentors ezinkulu zetanki enzulu, kubandakanya nezixhobo ezisezantsi zokubuyisela nokucoca. Uxinzelelo lomhluzi wokubilisa kunye ne-viscosity zihlala zijongwa kwaye zilawulwa kusetyenziswa izinzwa zenkqubo ezikwi-inline, abalawuli beemodeli zangaphakathi, kunye neenkqubo ezizenzekelayo ukuphucula isivuno kunye nokuthintela ungcoliseko.
Iimpawu zePenicillium Chrysogenum kwiBatch Fermentation ukuze kuveliswe iPenicillin
I-Penicillium chrysogenum, i-filamentous fungus, yeyona microorganism ikhethwayo kwimveliso ye-penicillin kwimizi-mveliso. Indlela esebenza ngayo ngexesha lenkqubo yokubilisa iyinkimbinkimbi kakhulu kwaye ichaphazela ngokuthe ngqo ukusebenza kakuhle kunye nemveliso yokugqibela. Ukuqonda iimpawu zayo kubalulekile ekulawuleni inkqubo ngempumelelo.
Ngexesha lokubilisa, i-viscosity kunye noxinzelelo zidibene ngokusondeleyo, kwaye utshintsho kwenye luchaphazela ngqo enye. Olu lwalamano lubaluleke kakhulu kulawulo lwenkqubo, njengoko luchaphazela yonke into ukusuka ekukhuleni kweentsholongwane ukuya kwimveliso.
Utshintsho lwe-Viscosity
Ubukhali bomhluzi wokubilisa buyindlela yokulinganisa ukumelana kwawo nokugeleza kwamanzi. Awuhlali unjalo kwaye uhlala utshintsha kulo lonke umjikelo wokubilisa ngenxa yezizathu ezahlukeneyo:
Uxinaniso lwee-microorganisms luyanda ngokukhula kwazo kwaye luphindaphindeka ekukhuleni kwe-biomass. Ubukho nje obubonakalayo budala uthungelwano oluxineneyo lweeseli, oluphakamisa kakhulu i-viscosity yomhluzi wokubilisa.
I-cell lysis yenzeka ekupheleni kokubila. Iintsholongwane zinokubila ukuba iiseli zixinezelekile, zikhuphe izinto ezingaphakathi kwiseli ezifana ne-DNA kunye neeproteni. Ezi molekyuli zinkulu zinokunyusa kakhulu i-viscosity yomhluzi.
Utshintsho kwiNgqwalasela
Uxinaniso luyatshintshatshintsha xa iibhaktheriya ziphindaphindana njengoko uxinano lweeseli lubangela umhluzi oxineneyo kunye nokusebenzisana rhoqo phakathi kweeseli. Kakade ke, uxinaniso lokuqala lwezinto ezinje ngeeswekile kunye nezondlo lunefuthe elicacileyo ekukhuleni ngakumbi kweentsholongwane.
Iiproteni ezikhutshiweyo kunye ne-polysaccharide ziyaqokelelana xa zivuthwa rhoqo, nto leyo enegalelo ekwandeni koxinzelelo lwezinto ezinyibilikisiweyo okanye ezixhonyiweyo.
Impembelelo yoBudlelwane
Ubudlelwane obusondeleyo phakathi kwe-viscosity kunye noxinzelelo bunempembelelo enkulu kwinkqubo yokubilisa. Ukunyuka kwe-viscosity ngenxa yoxinzelelo oluphezulu kungenza oku kulandelayo:
Ukuthintela Ukudluliselwa Kwezinto Ezininzi: Ubuninzi be-viscosity benza kube nzima ukuhambisa iigesi ezibalulekileyo ezifana ne-oksijini ezivela kumaqamza omoya ziye kumhluzi, nto leyo ethintela amandla okukhula kweentsholongwane.
Nciphisa Ukusebenza Kokuxuba: Umhluzi otyebileyo udinga amandla angakumbi ukuze uxube kakuhle. Oku kunokukhokelela kwiindawo ezimileyo apho izondlo ziphela khona kwaye inkunkuma iqokelelana, nto leyo edala imeko-bume engalinganiyo echaphazela kakubi imveliso.
Ichaphazela ukucubungula okungaphantsi: Imihluzi exineneyo kakhulu kunzima ukuyihluza, ukuyijika ibe yi-centrifuge, kunye nokuyicoca ngexesha lokubuyiselwa kwemveliso, nto leyo eyenza yonke le nkqubo ingasebenzi kakuhle kwaye ibize kakhulu.
Ngoko ke, ukujonga ubungqindilili kunye nokugxila ngexesha langempela kubalulekile ekugcineni iimeko ezifanelekileyo, ukuqinisekisa umgangatho wemveliso ohambelanayo, kunye nokwandisa isivuno.
Izicelo zoBeko-liso lwe-Inline Viscosity
Amandla okuguqula indlela yokubeka esweni kwi-intanethi kwinkqubo yokubilisa ibonakala ngakumbi kwimveliso yee-antibiotic, njenge-penicillin. Ukulandelela i-viscosity ngexesha langempela kuvumela abaqhubi ukuba balinganise ukukhula kweentsholongwane kunye nokuhambelana komhluzi, okuvumela uhlengahlengiso oluchanekileyo kwiindlela zokutya, umoya, kunye nokushukuma.
Iitekhnoloji zenkqubo ezizenzekelayo zisebenza njengesilumkiso phambi kokuba kubekho izinto ezingaqhelekanga, ungcoliseko, ukwakheka kwegwebu ukuthintela iingozi zemveliso. Ngaphezu koko, ivumela ukwandiswa ukusuka kwilabhoratri ukuya kwi-bioreactors yemizi-mveliso.
Izisombululo zokulinganisa i-Lonnmeter Inline Viscosity
Imisebenzi ephambili kunye neenkcukacha zobugcisa
I-inline sanitary viscometer kunyeimitha yoxinano lwamayezaZenzelwe ukujonga ukubiliswa kunye nokulawula inkqubo kwizicelo zamayeza. Uyilo lwazo olukhethekileyo loomatshini lunciphisa amathuba okuqokelelana kweebhaktheriya, ukugcinwa rhoqo kunye nokutshintshwa.
Eziizixhobo zokwenza izinto ngokuzenzekela ngaphakathiibonelela ngemilinganiselo ye-viscosity kunye ne-density yexesha langempela, iqinisekisa iimeko ezifanayo zomhluzi kwi-bioreactors. I-viscometer ye-inline sanitary inoluhlu lwe-viscosity oluphakathi kwe-10–10000,000 cP. Ukunyamezela kwayo ubushushu kufikelela kwi-350°C.
Zixhotyiswe ngee-sensors eziphambili ezisekelwe ekudlidlizeni okanye ekuphenduleni, ezi zixhobo zibonelela ngokufundwa okuchanekileyo kwiimeko eziguquguqukayo kwaye zidibana kakuhle neenkqubo ze-PLC kunye ne-DCS zolawulo oluzenzekelayo, nto leyo ezenza zibe zilungele inkqubo yokubilisa i-penicillin.
Iingenelo ngaphezu kweendlela zendabuko ezingaxhunyiwe kwi-intanethi
Izixhobo zokulinganisa i-viscosity ezikwi-inline zaseLonnmeter zigqwesa iindlela zemveli ezingaxhunyiwe kwi-intanethi. Izixhobo ze-automation eziphucukileyo zenkqubo zenza ukuba kubekho uhlengahlengiso lwenkqubo olukhawulezileyo, zisuse iimpazamo zesampulu.
Ukuchaneka kwazo ekuphatheni imihluzi engeyiyo eyeNewtonian kudlula izixhobo ezingasebenziyo, ezihlala zingasebenzi kakuhle xa zidibene nolwelo oluntsonkothileyo. Uyilo lococeko lunciphisa iingozi zongcoliseko, olubaluleke kakhulu kwiindawo zamayeza, ngelixa ukuqokelelwa kwedatha ngokuzenzekelayo kunciphisa iimpazamo zabantu, kuqinisekisa ukuthembeka okuphezulu kokujonga inkqubo yokubilisa kwi-intanethi.
Iingenelo kwi-Automation Process Process Automation
Izisombululo zeLonnmeter ziguqula ukwenziwa kwezinto ngokuzenzekelayo ekuvuthweni okuqhubekayo kwepenicillin, zibonelela ngeenzuzo ezingenakuthelekiswa nanto. Ziqinisekisa ukuba umhluzi wokuvutshelwa uhlala uthambile, nto leyo ephumela kwimveliso efanayo yepenicillin kunye nobunyulu obuphezulu.
Ulawulo lwe-viscosity oluzenzekelayo lunciphisa inkunkuma, ukusetyenziswa kwamandla, kunye nexesha lokungasebenzi, nto leyo eqhuba ukonga okukhulu kweendleko. Idatha yexesha langempela ivumela ukulungiswa kakuhle kwamazinga okutya, umoya, kunye nokuphazamiseka, nto leyo ephucula ukusebenza kakuhle kwenkqubo.
Ukubaluleka okubalulekileyo kokujonga i-inline viscosity ekuvuthweni kwamayeza, ingakumbi kwinkqubo yokuvutshelwa kwe-penicillin, akunakugxininiswa kakhulu. Yisa inkqubo yakho yokuvutshelwa kwinqanaba elilandelayo—nxibelelana neLonnmeter namhlanje ukuze ucele ikowuteshini eyenzelwe wena yezisombululo zethu zokulinganisa i-viscosity eziphambili kwaye uvule ukuchaneka kwemveliso okungenakuthelekiswa nanto!
Ixesha leposi: Agasti-14-2025
